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食品研究与开发:2017,38(9):122-126
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生物酶法提取黄秋葵果实黄酮工艺优化研究
王维婷1,2,曹宏杰3,郭溆1,2,程安玮1,2,刘超1,2,孙金月1,2,*
(1. 山东省农业科学院 农产品研究所,山东 济南 250100;2. 山东省农产品精深加工技术重点实验室, 山东 济南 250100;3. 青岛农业大学 食品科学与工程学院,山东 青岛 266109)
Optimization of Bio-enzyme Method for Flavones Extraction from Okra Fruit
WANG Wei-ting1,2, CAO Hong-jie3, GUO Xu1,2, CHENG An-wei1,2, LIU Chao1,2, SUN Jin-yue1,2,*
(1. Institute of Agro-Food Science & Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China; 2. Key Laboratory of Agro-Products Processing Technology of Shandong Province, Jinan 250100, Shandong, China; 3. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, Shandong, China)
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投稿时间:2016-07-18    
中文摘要: 为优化黄秋葵果实黄酮的提取工艺,以黄秋葵果实为原料,采用生物酶(纤维素酶、果胶酶、木瓜蛋白酶)法提 取黄秋葵果实黄酮,探讨酶添加量、酶解温度、料水比、酶解时间、缓冲液 pH 值对黄酮得率的影响。在单因素试验的基础 上,设计正交试验对黄秋葵果实中黄酮的提取工艺条件进行优化;最优条件下,纤维素酶、果胶酶、木瓜蛋白酶提取黄秋 葵果实黄酮最高含量分别为:70.385、64.522、62.167 mg/g。
中文关键词: 黄秋葵  黄酮  果胶酶  纤维素酶  木瓜蛋白酶
Abstract:In order to optimize the fruit extraction technology of falconoid, bio-enzyme method was applied for total flavones extraction from okra fruit. The impact of flavones of enzyme concentration,pH,temperature,mass ratio of material to water,time had been discussed. Basing on the single factor experiments, orthogonal design experiments had been used to optimized the extraction condition. However, the okra fruit highest flavonoid con- tent could reached to 70.385, 64.522, 62.167 mg/g using the cellulase, pectinase, papain extraction under the optimal conditions.
keywords: okra  flavonoids  pectinase  cellulase  papain
文章编号:201709027     中图分类号:    文献标志码:A
基金项目:山东省农业科学院青年科研基金项目(2014QNM55);山 东省自主创新成果转化专项(2014CGZH0712)
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