Abstract:To research the extract process of total flavonoids in Lycium barbarum L. with the enzyme-microwave extraction method. The effects of ethanol concentration， enzymolysis temperature and enzymolysis pH on the ex－ traction rate of total flavonoids were studied by orthogonal experiment based on single factor experiment. Then enzyme hydrolysate were extracted by microwave method on the optimum technological parameters determined by single factor experiment and orthogonal experiment. The optimum extraction conditions of enzymatic hydroly－ sis phase was ethanol concentration 45 %， temperature 50 ℃， pH4.0. The optimum extraction conditions of mi－ crowave phase was liquid -solid ratio 30 mL/g， temperature 65 ℃ ， time 4 min. The extraction rate of total flavonoids in Lycium barbarum L. was 1.560 % under the optimum extraction conditions of cellulase-microwave extraction method .