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食品研究与开发:2017,38(9):79-84
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响应面法优化枸杞水溶性蛋白提取工艺
于士婷1,尹翌秋2,孙天霞1,张丽轩1,王敏1,赵大庆1,王思明1,*
(1. 长春中医药大学,吉林 长春 130117;2. 长春金赛药业有限责任公司,吉林 长春 130012)
Optimization of Water-soluble Protein Extraction Technology of Wolfberry by
YU Shi-ting1, YIN Yi-qiu2, SUN Tian-xia1, ZHANG Li-xuan1, WANG Min1, ZHAO Da-qing1, WANG Si-ming1,*
(1. Changchun University of Chinese Medicine,Changchun 130117, Jilin, China; 2. Gene Science Pharmaceuticals Co., Ltd., Changchun 130012, Jilin, China)
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投稿时间:2016-10-21    
中文摘要: 通过响应面法(RSM)确定枸杞水溶性蛋白最佳提取工艺。 以枸杞蛋白提取率为指标,通过单因素试验考察各 个影响因素对枸杞蛋白提取率的影响程度。在此基础上,应用响应面分析法(RSM)优化提取供工艺条件,确定最佳提取 工艺。依据 design expert8.0.6 试验软件回归分析确定提取时间对枸杞水溶性蛋白提取率影响较大,分析得出枸杞水溶性 蛋白提取的最佳工艺参数为 pH8.60,液料比 18.83 ∶ 1(mL/g),提取时间 9.79 h。此条件下枸杞水溶性蛋白理论提取率7.82 %。但考虑到此参数给实际操作带来了诸多不便,故把枸杞水溶性蛋白的提取最佳工艺条件修正为 pH9.0 , 提取时 间 10 h, 液料比 20 ∶ 1(mL/g)。对修正参数进行 3 次重复验证试验得枸杞水溶性蛋白的实际提取率为 7.69 %,与预测值 接近。
中文关键词: 枸杞  枸杞水溶性蛋白  提取  响应面法
Abstract:To determine the optimum extraction wolfberry water-soluble protein by response surface methodol- ogy (RSM). Wolfberry protein extraction rate by single factor experiment investigated the influence of various factors wolfberry protein extraction rate. On this basis, the application of response surface methodology(RSM) for optimization of extraction conditions, to determine the best extraction process. According to the design expert 8.0.6 testing software regression analysis identified a greater impact on the extraction time wolfberry water-solu- ble protein extraction rate, the analysis of water-soluble proteins derived wolfberry extract the optimum param- eters pH8.60, liquid-solid ratio 18.83 ∶ 1(mL/g)extraction time 9.79 h. Wolfberry water-soluble protein extrac- tion rate of 7.82 % theory this condition. But consider this argument to practice a lot of inconvenience, so the water-soluble protein extract wolfberry optimum conditions corrected to pH9.0, extraction time 10 h, liquid-solid ratio 20 ∶ 1(mL/g). Amendments parameter verification experiment was repeated 3 times the actual extrac- tion rate of wolfberry water-soluble proteins 7.69 %, close to the predicted value.
文章编号:201709018     中图分类号:    文献标志码:A
基金项目:吉林省中药生物技术重点实验室(20140622003JC);吉林 省中药组学工程实验室(2014N155)
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