Abstract:The content of amino acids in different parts of Stropharia rugoso-annulata was analyzed in this pa－ per. Amino acids pattern spectrum method and amino acid ratio coefficient method were used to evaluate the nutrition. The results showed that Stropharia rugoso-annulata include 18 amino acids， which were valine， glu－tamic acid， aspartic acid， methionine， phenylalanine， leucine and threonine， and so on. The percentage of to－ tal amino acid contents in Stropharia mushroom cap and mushroom stem were 17.51 % and 6.67 %， respective－ ly， which contained the human body needs 8 essential amino acids. The protein content in Stropharia mushroom cap and mushroom stem was close to the egg protein （protein standard）， the close degree were 0.584 2 and 0.426 7， respectively. The first limiting amino acids is leucine and the amino acid ratio coefficient were 71.38 and 36.33 in Stropharia mushroom cap and mushroom stem， respectively. Nutritional value evaluation showed and that mushroom cap E/（E+N） and E/N in Stropharia mushroom cap and mushroom stem were 0.41 and 0.46， 0.69 and 0.85， respectively， which is close to the FAO/WHO reference protein pattern. All of the results showed that Stropharia has high value in medicine and nutrition science， whichis a worthy and vigorously develop resources.