Abstract:Rhodiola was used as raw material to prepare a diet rich in rhodiola extract， which was applied to prevent and relieve the fatigue of plateau sport. The results showed that the anti-fatigue related cellular path－ ways were activatedin mouse skeletal muscle cells， and the expression of related proteins was significantly high－ er than that in the control group（P<0.05）. It was confirmed that the extract of rhodiola had obvious anti-altitude exercise fatigue effect at the cellular level.Human volunteers plateau motion experiments confirmed that the ex－ ercise -related indexes and fatigue -related biochemical indexes in the experimental group were significantly higher than those in the control group （P<0.05）， and no adverse events were observed. These results showed that rhodiola was effective in anti-plateau fatigue and had certain application value.