Abstract:As Cordyceps militaris and iron element have been proved to have important physiological functions to human body. Through designing the single factor and multi-factor orthogonal experiments to explore the opti－ mization of submerged fermentation condition of iron-riched Cordyceps militaris. The best culture medium for－ mula of submerged fermentation had been determined by experiments as follows ： soybean meal 35 g/L， sugar 30 g/L， KH2PO4 1.5 g/L， MgSO4 1.5 g/L， thiamine 5×10-5 g/L， FeSO4·7H2O 0.3 g/L. The best culture condition had been achieved as follows： pH6， temperature 26 ℃， fermentation 3 days， inoculation quantity 5 %， fluid volume 100 mL/250 mL of triangle flask. Under the optimal conditions of the submerged fermentation， it was measured that the biomass of iron-riched Cordyceps militaris was 18.93 g/L， and the enrichment of iron was 16.01 mg/g.