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投稿时间：2016-07-18
投稿时间：2016-07-18
中文摘要: 为了研究食甲醇芽孢杆菌（Bacillus methylotrophicus）J4-1 生物转化对山楂提取物挥发性成分的影响，采用同时 蒸馏萃取、GC/MS 对比分析经回流提取后由 J4-1 转化的山楂提取物和对照的挥发性成分。结果表明：①微生物转化山 楂提取物鉴定出 58 种挥发性成分，新增 35 种，包括己酸、乙酸己酯、丁酸己酯、松油醇、二氢猕猴桃内酯、光黄素等，占 总相对百分含量 21 %以上；对照样品鉴定出 34 种挥发性成分；②相对百分含量显著增加的有己醛、糠醛、葵醛、棕榈酸 乙酯、柠檬酸三丁酯等 15 种，刺激性成分如邻苯二甲酸异丁酯、邻苯二甲酸单（2-乙基己基）酯显著降低，14 种挥发性 成分消失。经微生物转化的山楂提取物挥发性成分改变大，具有酸甜、奶香丰富香气，刺激性成分减少，香气质提高。
中文关键词: 食甲醇芽孢杆菌（J4-1） 微生物转化山楂提取物 GC/MS 挥发性香气成分
Abstract:To Study the influence of biotransformation on the volatile components of hawthorn extract， the con－ stituents in the biotransformed hawthorn extract and its contrast were analyzed by GC/MS after simultaneous distillation. Results： ①58 volatile components were identified from the biotransformed hawthorn extract， in－ cluding 35 kinds of new： hexanoic acid，acetic acid ester，hexyl butyrate，terpinol，dihydroactinidiolide，lumi－ flavine， etc.， which accounted for 21 % of the total volatile components of the biotransformed hawthorn extract； and 34 kinds of volatile components were identified from the control samples； ②15 kinds of volatile compo－ nents' relative percentage content increased significantly， including hexanal，furfural，decanal，hexadecanoic acid， ethyl palmitate，tributyl citrate， etc.；Irritating ingredients such as 1，2-Benzenedicarboxylic acid， butyl 2-methylpropyl ester and 1，2-Benzenedicarboxylic acid， mono （2-ethylhexyl） ester decreased significantly； 14 kinds of volatile components disappeared. After biotrasformation， the volatile components of hawthorn extract has changed greatly， with sweet and sour， milk fragrance rich aroma， and stimulating ingredients reduce、im－ proving the quality of aroma.
keywords: Bacillus methylotrophicus （J4 -1） biotransformed hawthorn extract GC/MS volatile aroma components
文章编号：201707033 中图分类号：10.3969/j.issn.1005-6521.2017.07.033 文献标志码：A
基金项目:
| Author Name | Affiliation |
| CHEN Yi-kun， LUO Cheng-hao， WEI Min*， LI Ran， YUE Hai-bo， GUO Guo-ning | Technology Center of China Tobacco Hubei Industry Limited-liability Company， Wuhan 430040， Hubei，China |
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