Abstract:Glucosinolates（GLs）， an important kind of secondary metabolite in broccoli， can be hydrolyzed into several bioactive components through endogenous myrosinase. GLs and their degradation products play an im－ portant role in anti-cancer， anti-oxidation and anti-microbial， which have drawed a great deal of attention of reasearchers. In this paper， the strucultural propertites， synthesis and degradation pathway of GLs were summa－ rized， the seperation and detection technologies， physiological function of GLs were reviewed.which benefits for rational exploitation and utilization of GLs. This may provide a scientific basis reference for further studies con－ cerning rational explorations and utilizations of GLs.