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投稿时间：2016-07-23
投稿时间：2016-07-23
中文摘要: 以沧州小枣蛋白液为原料，水解度和多肽得率为检测值，先进行单因素试验，通过 spss 相关分析选出最显著的3 个因素：加酶量、酶解时间和酶解温度，然后以酶解多肽得率为评价指标用响应面分析法优化酶解工艺，结果表明，最 佳酶解条件为加酶量 5 000 U/g，酶解温度 55 ℃，酶解时间 5 h，在此条件下，小枣的酶解多肽得率达到了 55 %，与预测值 相近。
Abstract:Peptides were prepared by enzymatic hydrolysis of Cangzhou jujube.The effect of enzymolysis power， time and temperature were identified by the combined use of single factor method and spss analysis as factor with the most significant influence on peptides yield of protein hydrolysate.The optimal hydrolysis conditions were determined by response surface methodlogy to be 5 000 U/g，55 ℃，5 h，respectively.Under these condi－ tions，the predicted peptides yield was up to 55 %，agreeing with the average experimental value.
keywords: cangzhou jujube response surface methodology peptides enzymatic hydrolysis collaborative enzyme
文章编号：201706020 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| LIU Jia-kun， JIAO Yan，YU Yong-jun* | Cangzhou Medical Colledge， Cangzhou 061000， Hebei， China |
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