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投稿时间：2016-05-25
投稿时间：2016-05-25
中文摘要: 以胞外多糖产量和菌丝体干重为检测指标，优化黑木耳菌 Aas1502 胞外多糖液态深层发酵培养基组成。通过 单因素试验，确定培养基碳源和氮源种类及培养基中碳源、氮源、KH2PO4 和 MgSO4·7H2O 的浓度。利用正交试验进一步 优化，并通过方差分析最终确定优化培养基组成为：马铃薯淀粉 20 g/L、葡萄糖 20 g/L、蛋白胨 4 g/L、KH2PO4 1.5 g/L、 MgSO4·7H2O 0.2 g/L，此时胞外多糖产量和菌丝体干重，分别为 9.36 g/L 和 12.27 g/L，胞外多糖比优化前提高了 1.48 倍， 为后续利用深层发酵提取黑木耳多糖提供了理论基础。
Abstract:The composition of submerged fermentation medium for extracellular polysaccharides from Auricu－ laria Auricula strain 1502 was optimized in this study. Production of extracellular polysaccharide and mycelium dry weight were regarded as research index to determine the composition of the medium. Carbon sources， nitro－ gen sources， carbon concentration， nitrogen concentration， KH2PO4 concentration and MgSO4·7H2O concen－ tration were determined by single-factor test. Orthogonal test was used to analysis the variance of the two index－ es， the optimal composition of culture medium were determined： potato starch 20 g/L， glucose 20 g/L， peptone 4 g/L， KH2PO4 1.5 g/L and MgSO4·7H2O 0.2 g/L. Production of extracellular polysaccharide and mycelium dry weight were 9.36 g/L and 12.27g/L， respectively. The effect was significant that the production of extracellular polysaccharide was 1.48 times as before. A theoretical basis was provided for the subsequent extraction of polysaccharides from Auricularia Auricula strain using submerged fermentation by this result.
文章编号：201705042 中图分类号： 文献标志码：A
基金项目:吉林省重大科技攻关项目（20140203019NY）
| 作者 | 单位 |
| 刘俊梅1，丁伟1，王庆1，杨盼盼1，王玉华1，朴春红1，于寒松1，李琢伟2，* | 1.吉林农业大学 食品科学与工程学院，吉林 长春 130118； 2. 长春职业技术学院，吉林 长春 130033 |
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