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投稿时间：2016-12-09
投稿时间：2016-12-09
中文摘要: 为研究黑曲霉产黄曲霉毒素 B1（AFB1）降解酶的影响因素，试验以 AFB1 降解率作为评价指标，考察不同培养基 成分和不同发酵条件对黑曲霉产 AFB1 降解酶的影响。结果显示，碳源、氮源、金属离子、AFB1 类似物等对黑曲霉生长影 响不大，但对降解酶合成有显著影响，且该酶是一种诱导酶；发酵温度、时间、初始 pH 等对降解酶合成也有显著影响，当 发酵温度为 30 ℃时 AFB1 降解率达 87.5 %，初始 pH 为 7.0 时 AFB1 降解率达 89.3 %，发酵时间与 AFB1 降解率呈正相关 性，培养 4 d 时降解率达 83.5 %。研究结果可为 AFB1 降解酶的发酵工艺条件优化及工业化生产提供理论指导。
Abstract:To study the effect of different factors on the production of aflatoxin B1 （AFB1） degradation enzyme from Aspergillus niger. Experiment with AFB1 degradation rate as the evaluation index， inspected different culture medium and different fermentation conditions.The results showed that the carbon and nitrogen sources， metal ions and AFB1 analogs had little effect on the growth of Aspergillus niger， but had significant effect on the synthesis of degradation enzyme， and the enzyme was an inducible enzyme. Furthermore，fermentation tempera－ ture， time and initial pH had significant effects on the degradation of enzyme synthesis. When the temperature （30 ℃） and the initial pH 7， the degradation rate of AFB1 reached 87.5 % and 89.3 %， respectively. The re－search also found that fermentation time and the degradation rate of AFB1 was positively correlated. On the days 4， degradation rate of AFB1 was 83.5 %. The research results can provide theoretical guidance for the optimiza－ tion of fermentation conditions and industrial production of AFB1 degrading enzyme.
文章编号：201705041 中图分类号： 文献标志码：A
基金项目:陕西省科技厅自然基金项目（2013JQ3019）；陕西省农业科技创新与攻关项目（2015NY002）
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