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食品研究与开发:2017,38(3):60-65
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黑果腺肋花楸黄酮提取工艺优化及体外抗氧化研究
刘静,徐莉莉
(内蒙古商贸职业学院 食品工程系,内蒙古 呼和浩特 010070)
Study on Black Fruit Aronia Flavonoids Extraction Process and Its Antioxidant Activity
LIU Jing, XU Li-li
(Department of Food Engineering, Inner Mongolia Business & Trade Vocational College, Huhhot 010070, Inner Mongolia, China)
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投稿时间:2016-09-04    
中文摘要: 应用黑果腺肋花楸作为原料,采用响应面法优化黑果腺肋花楸黄酮提取工艺,并利用 VC 做对照试验,对其还 原力及以及·OH、O2-·、DPPH 自由基清除力进行研究。结果表明,最佳工艺参数为乙醇浓度 77.67 %、提取时间 81 min、料 液比 1 ∶ 20(g/mL),在此条件下,黑果腺肋花楸黄酮得率 5.14 %。影响黑果腺肋花楸黄酮得率因素由大到小依次为乙醇 浓度>提取时间>料液比。 最佳工艺参数提取的黑果腺肋花楸黄酮对·OH、O2-·、DPPH 自由基的 IC50 分别为 3.27、3.96、 3.79 mg/mL,最大清除率达 29.58 %、70.23 %、61.41 %,并且其还原能力强,能够说明其体外抗氧化活性较强。
中文关键词: 黑果腺肋花楸  黄酮  响应面  抗氧化
Abstract:Black fruit Aronia as raw material, single factor and response surface analysis black fruit Aronia the extraction process, and VC as control its antioxidant capacity and 1,1-diphenyl-2 trinitrobenzene hydrazine rad- ical(DPPH·), superoxide anion radical (O2-·), hydroxyl radical (·OH)scavenging were studied. The results showed that the optimum parameters for the ethanol concentration 77.67 %, extraction time 81 min, 1 ∶ 20(g/mL), in this condition, black fruit Aronia flavonoids yield an average of 5.14 %. Effect of black fruit Aronia yield of flavonoids descending factor ethanol concentration> extraction time> solid-liquid ratio. The optimal parameters extracted black fruit Aronia flavonoids on·OH, O2-·, DPPH radical IC50 were 3.27, 3.96, 3.79 mg/mL, the maximum clearance rate of 29.58 %, 70.23 %, 61.41 % and showed a strong reducing power, has strong an- tioxidant activity in vitro.
文章编号:201703014     中图分类号:    文献标志码:A
基金项目:内蒙古商贸职业学院项目(NSZY1103)
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