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投稿时间：2016-05-05
投稿时间：2016-05-05
中文摘要: 为了进一步优化迷迭香抗氧化剂的提取方法，采用响应面法分析回流提取和超声辅助提取对迷迭香粗提物中 总酚含量的影响，并通过体外抗氧化实验来评价其抗氧化活性。结果表明，回流法提取迷迭香的最佳工艺条件为：乙醇 浓度 80 %，温度 80 ℃，时间 1 h，料液比 1 ∶ 10（g/mL），得到总酚含量为 33.85 mg/g。超声辅助提取迷迭香的最佳工艺条件 为：温度 50 ℃，时间 40 min，料液比 1 ∶ 10（g/mL），乙醇浓度 80 %，得到总酚含量为 36.58 mg/g。超声辅助提取物的抗氧化 效果优于回流提取物。
Abstract:In order to optimize the method for extracting rosemary antioxidant， response surface methodology was used to analyze the effect of reflux extraction and ultrasonic assisted extraction on the content of total pheno－ lic compounds in crude extract of rosemary. The antioxidant activities of the crude extraction were evaluated in vitro antioxidant experiment. The results indicated that the highest extraction yield of total phenolic compounds using the reflux extraction could obtain up to 33.85 mg/g under the following conditions： ethanol concentration of 80 % as solvent， liquid to solid ratio of 1 ∶ 10 （g/mL）， extraction for 1h at the temperature of 80 ℃. The highest extraction yield of total phenolic compounds using the ultrasound-assisted extraction could obtain up to 36.58 mg/g under the following conditions： ethanol concentration of 80 % as solvent， liquid to solid ratio of 1 ∶10（g/mL）， extraction for 40 min at the temperature of 50 ℃.The antioxidant effect of ultrasonic-assisted ex－ traction was better than that of reflux extraction.
keywords: Rosemary response surface antioxidant
文章编号：201703013 中图分类号： 文献标志码：A
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