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投稿时间:2015-12-31
投稿时间:2015-12-31
中文摘要: 本文以小黄鱼内脏为研究对象,从中分离提取出活性蛋白。通过对缓冲液 pH,提取时间,醇液比 3 个单因素的 考察,确定因素水平,并设计正交试验,对工艺进行优化,得出最佳提取工艺。试验结果表明最佳提取工艺为:提取时间 5 h,缓冲液 pH9,醇液比 0.8 ∶ 1(体积比),在此工艺下所提取的蛋白液浓度为 3.409 mg/mL。最后,用所提取的活性蛋白进 行初步应用性试验,通过美拉德反应,制备食品添加剂,为小黄鱼下脚料的应用提供了理论基础。
Abstract:The small yellow croaker visceral as the research object, designing the extraction process, separat- ing and extracting the active protein from the small yellow croaker visceral. Determining the factor level through the investigation of three single factor, buffer pH, extraction time and alcohol liquid, to optimize the process and obtain the best extraction process by designing the orthogonal experimental L9(33). The results showed that: 5 h of extraction time, 9 of buffer pH, 0.8 ∶ 1 (volume ratio)of alcohol liquid ratio made the best extraction,in this process the extracted an average of 3.409 mg/mL. Finally, with the extraction of active protein applied ex- periments, through maillard reaction, the preparation of food additives, provides theoretical basis for the appli- cation of small yellow croaker scraps.
keywords: Pseudosciaena polyactis fish offa active protein
文章编号:201703012 中图分类号: 文献标志码:A
基金项目:舟山市科技计划项目(2014C11009);浙江海洋学院科研 启动项目(21025011013)
作者 | 单位 |
吴凯强1,姜维2,郭健1,应知伟3,宋文东2,* | 1. 浙江海洋学院 食品与医药学院,浙江 舟山 316022;2. 浙江海洋学院 创新应用研究院,浙江 舟山 316022;3. 浙江海洋学院 海洋科学与技术学院,浙江 舟山 316022 |
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