Abstract:To observe the effect of blueberry anthocyanins on scopolamine-induced learning and memory impairment in mice. Sixty male KM mice were randomly divided into 6 groups：a blank control group，a model control group ，a positive control group（ laci staw 200 mg/kg· d），a high-dose blueberry anthocyanins group（400 mg/kg· d），a middle-dose blueberry anthocyanins group（200 mg/kg· d） and a low-dose blueberry anthocyanins group （100 mg/kg·d）.Each group was continuously administered 14 days. On the15th day，except for the mice in blank group， other mice were injected scopolamine 2 mg/kg to establish memory impairment model.The step-down passive avoidance test was performed after the last administration of 2 hours. After testing，all mice were beheaded to detect the effect of AchE. The expression of NF-kB p65 and Cleaved caspase-3 in the hippocampus of mice was detected by Western blotting. The results showed that：the blueberry anthocyanins ineach dose group could significantly increase the incubation period platform in mice，decreased the mistaken re-action times in 5 min（P<0.05），and could reduce AChE activity in the cerebral cortex and hippocampus （P<0.05）.The result of Western blotting showed that blueberry anthocyanins could significantly reduce the expression of NF-kB p65 and Cleaved caspase-3 protein in the hippocampus of scopolamine-induced mice. Blueberry anthocyanins have certain improving effects on memory impairment induce by scopolamine in mice model of learning and memory disorder.