Abstract:The liquid fermentation condition for the Paecilomyces sinensis was optimized in this essay in order to expand the usage of natural pigment. Firstly，the most suitable carbon source and nitrogen source was select原 ed to be glucose and peptone，respectively. Afterward，other conditions such as temperature，shaking speed， pH value were optimized by single factor experiment under the condition of using them as carbon and nitrogen source. The result showed that the optimum figure for temperature，pH and shaking speed were 25 益，neutral and 160 r/min ，respectively. Furthermore，response surface analysis was conducted to verify the results. The op原 timum figures showed less than 2 % variance in comparing with the theoretical response value。