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投稿时间：2016-07-22
投稿时间：2016-07-22
中文摘要: 建立一种用高效液相色谱-PDA 检测法测定运动饮料中甜菊糖苷的检测方法。 样品前处理采用硅胶固相萃 取小柱进行富集和净化，氮气吹干后用流动相复溶上机检测。采用 Waters RP C18 色谱柱（4.6 mm×250 mm，5 μm）分 离，以乙腈 ∶ 水=30 ∶ 70（体积比）作为流动相，用 PDA 检测器检测，外标法峰面积定量。结果表明，9 种甜菊糖苷组分在20.0 μg/mL~200.00 μg/mL 浓度范围内线性良好，相关系数 r2 为 0.991~0.999，检出限在 1.0 μg/mL~3.0 μg/mL，定量限 为 3.0 μg/mL~9.0 μg/mL，加标回收率达到 81.4 %~90.5 %。
Abstract:A rapid and effective method was established for the determination of steviol glycosides in sports drink by high performance liquid chromatography-PDA detection method. The samples were concentrated and purified by silica gel solid phase extraction column.The elution liquid was dried under nitrogen， and detected af－ ter re-dissolution with mobile phase. The separation of targeted compound was performed on a Waters RP C18 chromatographic column （4.6 mm×250 mm， 5 μm） using acetonitrile ∶ water （30 ∶ 70） as mobile phase， with PDA detector and external standard method peak area quantification. The linear range of nine kinds stevioside components was in the range of 20.0 μg/mL-200.0 μg/mL with a correlation coefficient of 0.991-0.999. The de－ tection limit was 1.0 μg/mL-3.0 μg/mL and the quantitative limit was 3.0 μg/mL-9.0 μg/mL. The recovery rate was 81.4 %-90.5 %.
文章编号：201701037 中图分类号： 文献标志码：A
基金项目:
| 作者 | 单位 |
| 周文清 | 景德镇陶瓷大学，江西 景德镇 333000 |
| Author Name | Affiliation |
| ZHOU Wen-qing | Jingdezhen Ceramic Institute， Jingdezhen 333000， Jiangxi， China |
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