Abstract:Food-borne pathogenic bacteria is the main factors that endanger food safety and it has done great harm to human health，which has received more and more widespread attention.So establishing simple and efficient detection methods is an important part of food hygiene and security detection.Aptamers are single-stranded oligonucleotides that bind to target molecules with high affinity and specificity，which are selected by SELEX from a synthetic random ssDNA pool.A variety of aptamers for the detection of food-borne pathogenic bacteria have been successfully screened out and applied.This paper will introduce detection methods of food-borne pathogenic bacteria baesd on aptamers and discuss its application process and development prospect.