Abstract:Study on the application of the amplification sequencing technology in the research of compound bacteria fermented beverage.Taking the Kombucha beverage as an example，throught the genomic DNA extraction in oral liquid of Kombucha，amplicon sequencing of 16S rDNA and ITS using the method of high-throughput sequencing of amplicons of sequencing to analysis and identification the dominant microbial in oral liquid of Kombucha.The classification and statistics of the species by the special species classification tree，finally got the main bacteria composition：Acetobacter was 89.11%，Bacteroides was 5.56%，Oscillospira was 1.77%.The main fungus composition：Yeast was 93.48%，of which Candida ethanolica was 61.76%，Blastobotrys adeninivorans was 30.97%.The result of Alpha Diversity analysis showed that：the sequencing data was more reasonable，the sequencing data amount was large enough，can reflect most of the microbial species information in the sample.The the application of the amplification sequencing technology has advantages in the analysis of the advantages of the compound bacteria fermented beverage and the detection of the content of probiotics.