Abstract:Targeting cytochrome b gene of mitochondrial DNA from beef，sheep，pork，chicken，duck，horse，the specific primers and TaqMan probes of sheep were designed.Combined with real-time PCR technology，the 16SrDNA reference gene was introduced，and the quantitative detection method of sheep in Fresh meat and meat products was established using reference gene correction by exogenous species-specific gene.The established method was validated through specific，universal detection，and analog mixed samples.The results showed a good specificity and a good universality.Through the construction of standard curve，both sheep-specific and universal systems showed a good linear relationship（R2＞0.996）.By Quantity value and the correction coefficient of sheep species-specific gene and 16SrDNA reference gene，the mass percentage content of the sample contained in sheep-derived ingredients could be calculated.The PCR method showed a high accuracy and the average recovery of sheep-derived ingredients in meat mixtures of simulation samples was 96.25%.