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投稿时间：2015-06-20
投稿时间：2015-06-20
中文摘要: 啤酒花是一种重要的风味剂，也是酿造啤酒的重要原料。本文采用紫外分光光度法测定了6种不同品牌啤酒中异-酸（iso-α-酸）的含量。试验考察了不同表面活性剂、介质酸度对iso-α-酸紫外吸收的影响。结果表明：iso-α-酸在258 nm处有较强的吸收，方法线性范围在10 μg/mL～50 μg/mL，相关系R=0.999 4，RSD=1.40%（n=5）。通过比较说明β-环糊精（β-CD）对iso-α-酸的增敏作用最强，SDS次之，曲拉通-100最弱。6种不同品牌啤酒中iso-α-酸的含量（μg/mL）依次为：司陶特黑啤酒（130.33）＞燕京啤酒（67.45）＞哈尔滨啤酒（58.14）＞崂山啤酒（39.62）＞纯生啤酒（38.76）＞雪花啤酒（35.53）。用标准加入法测定其回收率，样品回收率范围在96.30%～104.70%之间，结果满意。
Abstract:Hops is a kind of important flavour enhancer and raw material of brewing industry.In this article，iso-α-acid content in beer with 6 different brands were measure d through ultraviolet spectrophotometry.The effects of the different surfactants，medium acidity on absorption of α-acid were explored.The results showed that the α-acid had the strong absorption at 258 nm，the linear range of 10 μg/mL to 50 g/mL，correlation coefficent R=0.999 4，the RSD=1.40%（n=5），respectively.The sensitized action with different surfactants for α-acid absorption were as follows：β-CD，the SDS，Brij-35，TritonX-100 and CTMAB.The iso-α-acid contents（g/mL）in beer with 6 different brands were Si Tao Te Hei（130.33）＞Yanjing beer（67.45）＞Harbin beer（58.14）＞Laoshan beer（39.62）＞Yanjing pure draft beer（38.76）＞Snow Flower beer（35.53）.The average recoveries were in the range of 96.30%-104.70%with standard addition method，the results had been satisfied for sample determination.
keywords: beer iso-α-acid β-CD ultraviolet spectrophotometry
文章编号：201613023 中图分类号： 文献标志码：
基金项目:忻州师范学院重点学科建设项目资助计划（xk201402）；山西省教育厅重点建设学科项目（20141010）；山西省高等学校教学改革项目（2015-100）
| Author Name | Affiliation |
| CHEN Jin-e，HE Pei，ZHANG Hai-rong | Lab of Biochemical Analysis，Xinzhou Teachers College，Xinzhou 034000，Shanxi，China |
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