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投稿时间：2015-06-04
投稿时间：2015-06-04
中文摘要: 为提高蜜环菌产量，采用正交试验法对蜜环菌生长培养基中葡萄糖、酵母粉、维生素B13个组分的添加量进行优化，利用平板法分离出影响蜜环菌生长的主要杂菌并采用分子生物学的方法对污染菌株进行鉴定。试验结果表明：葡萄糖45 g/L、酵母粉9 g/L、维生素B10.05 g/L为最佳配方。分离出两株霉菌，经过鉴定，分别为橘青霉Penicillium citrinum t1、塔宾黑曲霉Aspergillus tubingensis t2。
中文关键词: 蜜环菌 正交试验 rDNA-ITS菌种鉴定 筛选
Abstract:In order to improve the yield of Armillaria，glucose，yeast powder，vitamin B1three components were optimized by the method of orthogonal experiment.The end of plate method was used to isolate the main bacterial and affect the growth of Armillaria strains of pollution by using molecular biology method.The results showed that the glucose 45 g/L，yeast powder 9 g/L，vitamin B10.05 g/L as the best formula.Two strains of the mold were isolated and identified，the results showed thatthe one was Penicillium citrinum t1，the other was Aspergillus tubingensis t2.
文章编号：201612036 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| LIZai-xin，TANG Wen-cai | College of Bioengineering，Sichuan University of Science and Engineering，Zigong 643000，Sichuan，China |
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