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投稿时间：2016-03-09
投稿时间：2016-03-09
中文摘要: 采用单因素试验法对肉桂内生细菌Pseudomonas sp.RGEB06转化肉桂醛合成肉桂醇的反应条件进行了研究。在改良M9液体培养基的基础上，以肉桂醛转化率、肉桂醇转化产率、肉桂醇选择性等为指标，考察了转化过程中的肉桂醛底物浓度、初始pH、转化温度、接种量、装液量、摇床转速、预培养时间、转化时间等因素对Pseudomonas sp.RGEB06转化肉桂醛合成肉桂醇的影响。结果表明：在转化温度30℃、初始pH 6.5、装液量100 mL/250 mL三角瓶、接种量20%、摇床转速150 r/min、预培养时间24 h、底物浓度2.64 mg/mL、转化时间24 h时，肉桂醇的转化率达到最大值88.08%，生成肉桂醇的选择性为94.21%，转化液中肉桂醇的浓度为2.36 mg/mL。
Abstract:Biotransformation conditions of cinnamaldehyde into cinnamic alcohol by an endophytic bacteria Pseudomonas sp.RGEB06 isolated from Cinnamomum cassia were studied by the single factor test method. Based on the modified M9 liquid medium，effects of cultural conditions on cinnamaldehyde conversion rate，yield and production selectivity of cinnamyl alcohol were studied in detail.The results of single factor experiments indicated thatthe suitable biotransformation conditions for the production ofcinnamylalcoholwere as followed：conversion temperature of 30℃，initial pH 6.5，liquid volume 100 mL/250 mL flask，inoculation amount20%，shaker speed 150 r/min，pre-culture time 24 h，substrate concentration 2.64 mg/mL，conversion time of24 h.Under the optimalconditions，the yield and production selectivity ofcinnamylalcoholwere 88.08% and 94.21%，respectively，and the finalconcentration ofcinnamylalcoholin the culture was 2.36 mg/mL.
文章编号：201612035 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（31460409）；广西高校食品安全与检测重点实验室资助项目
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