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投稿时间:2015-06-05
投稿时间:2015-06-05
中文摘要: 对乳酸乳球菌乳脂亚种BTQY-112增殖培养基进行优化研究。经试验确定乳酸乳球菌乳脂亚种BTQY-112最适培养基为:葡萄糖为5 g/L,乳糖为5 g/L,酪蛋白胨3.3 g/L,大豆蛋白胨3.3 g/L,鱼蛋白胨3.3 g/L,β-甘油磷酸二钠28.5 g/L,Tween-80 0.5 g/L,七水硫酸镁0.25 g/L,乙酸钠1.55 g/L,K2HPO40.83 g/L,柠檬酸钠0.62 g/L,抗坏血酸钠1.5 g/L。在此增殖培养基中经30℃,14 h培养活菌数提高了4.3倍。
Abstract:The optimization ofproliferation culture medium for Lactococcus lactis subsp.BTQY-112 was studied.The bestmedium was gotwith the composition of5 g/L glucose,lactose 5 g/L,casein peptone 3.3 g/L,soybean peptone 3.3 g/L,fish peptone 3.3 g/L,β-glycerol phosphate disodium salt 28.5 g/L,Tween-80 0.5 g/L,MgSO4·7H2O 0.25 g/L,sodium acetate 1.55 g/L,K2HPO40.83 g/L,sodium citrate 0.62 g/L,sodium ascorbate 1.5g/L.After cultivated in enrichmentmedium for 14 h at30℃,number oflive bacteria increased 4.3 times.
文章编号:201612037 中图分类号: 文献标志码:
基金项目:
作者 | 单位 |
王菲菲 | 包头轻工职业技术学院,内蒙古包头014045 |
Author Name | Affiliation |
WANG Fei-fei | Baotou Light Industry Vocational TechnicalCollege,Baotou 014045,Inner Mongolia,China |
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