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投稿时间：2015-06-03
投稿时间：2015-06-03
中文摘要: 建立HPLC波长切换法同时测定黑豆皮中原儿茶酸、表儿茶素含量的方法。固定相为shim-pack VP-ODS C18（250mm×4.6mm，5μm），流动相为乙腈-0.1%磷酸溶液，流速为1.0 mL/min，检测波长为260、280 nm，柱温35℃。原儿茶酸、表儿茶素分别在0.010 52 mg/mL～1.052 mg/mL（r=0.999 6）和0.012 24 mg/mL～1.224 mg/mL（r=0.998 9）浓度范围内与峰面积呈良好的线性关系，平均回收率分别为99.46%（RSD=1.08%，n=6）和99.06%（RSD=1.02%，n=6）。
Abstract:To establish an HPLC method of switching-wavelength simultaneous determination of the content for protocatechuic acid and epicatechin in Black soybean coat.The solid phase was shim-pack VP-DOS C18（250 mm 4.6 mm，5 μm）.The mobile phase was composed of acetonitrile-0.1%Phosphoric acid，The flow rate was1.0 mL/min.The detection wavelength was260 nm and 280 nm，and the temperature of column was 35℃. Good linear relationships were found within the range of 0.010 52 mg/mL-1.052 mg/mL（r=0.999 6）for protocatechuic acid and 0.012 24 mg/mL-1.224 mg/mL（r=0.998 9）for epicatechin.The average recovery was 99.46%（RSD=1.08%，n=6）and 99.06%（RSD=1.02%，n=6）.
keywords: black soybean coat protocatechuic acid epicatechin HPLC switching-wavelength content determination
文章编号：201611035 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| LIU Zi-ming，LI Guo-hua | Jilin Engineering Vocational College，Siping 136001，Jilin，China |
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