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食品研究与开发:2016,37(11):-
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亲水色谱法测定硝酸盐和亚硝酸盐方法研究
(1.东北农业大学食品学院,黑龙江哈尔滨150030;2.天津市食品研究所有限公司,天津301609)
Research of Determination of Nitrate and Nitrite by Hydrophilic Chromatography
(1.College of Food Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;2.Food Research Institute Co.,Ltd.,Tianjin 301609,China)
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投稿时间:2015-11-12    
中文摘要: 建立使亲水色谱柱同时测定硝酸盐和亚硝酸盐的高效液相色谱方法。样品匀浆后超声提取,高速离心处理,采用亲水色谱柱分离,乙酸铵和乙腈作为流动相进行等度洗脱,210 nm紫外检测。该方法简便快捷、准确可靠,成本低廉,适用于多种蔬菜中亚硝酸盐和硝酸盐的分析。
中文关键词: 蔬菜  硝酸盐  亚硝酸盐  亲水色谱
Abstract:The method to determination of nitrate and nitrite in vegetables was established by HILIC column. Vegetables samples were pre-treated by ultrasonic extraction and high-speed centrifugation,separated by HILIC column which used ammonium acetate and acetonitrile as the mobile phase,isocratic elution and UV detection at 210 nm.The method was simple,rapid,accurate and reliable,cheap,and suited forthe analysis of nitrite and nitrate in vegetables.
keywords: vegetables  nitrates  nitrites  HILIC
文章编号:201611034     中图分类号:    文献标志码:
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