Abstract:Using the phenol-sulfuric acid method，with glucose as the standard by UV spectrophotometer and comparison of phenol-sulfuric acid method（methodⅠ），the method in China Pharmacopoeia（methodⅡ）and the method improved（methodⅢ），the content of Lycium barbarum polysaccharide was detected under 485 nm to compare the advantages of the three methods.Experiments showed that the three methods standard curve cor relation coefficient were 0.930 9，0.991 8，0.996 8，RSD of light absorption value stability were 10.95%，2.13%，0.13%；the relative error of recovery rate of methodⅢwas small，which showed no significant difference.It meant that the methodⅢwas the best.