Abstract:Alkali-solution and acid-isolation method was used to extract protein from Cabernet Sauvignon grape seed to improve the utilization rate of grape seed，in which respones surface analysis was used to optimize extraction conditions.Based on the single factor experiments，the Central Composite design principles and Design-Expert.8.05 data ananlysis software were used to select four parameters of response surface design to optimize extraction process.The result showed the optimize extraction conditions：the concentration of NaOH 0.001 mol/L，extraction temperature 45℃，extraction time 40 min，liquid to solid ratio 1∶25（g/mL），the extraction value was 9.75 mg/g.