Abstract:The technology for silkworm pupa protein hydrolysis by double enzymatic（alkaline protease and papain）was studied．Single factor and orthogonal experiments were used to study the effects of the parameters such as temperature，amount of enzyme，substrate concentration，time，mass ratio of a1kaline protease and papain on the hydrolysis degree of silkworm pupa protein.The results showed that the temperature was the more important factor that affected the effect of hydrolysis，following substrate concentration and time and amount of enzyme and enzyme mass ratio，the optimal conditions were obtained with orthogonal experiments，as follows：the temperature was 55℃，the amount of enzyme was 3.0%，the substrate concentration was 3%，the time was 6 h and the enzyme mass ratio was 4∶1.The hydrolysis degree of silkworm pupa protein under these conditions could be 25.04%．