Abstract:A total of 71 strains of yeast were isolated from the rhizosphere soil of mango tree in Changjiang or－ chard of Hainan province. Through the method of confront culture ， the yeast strain named `LW' was screened out ， which had the most antagonistic effect to Colletotrichum gloeosporioides， the inhibition zone diameter of it was 12.25 mm. The vivo test showed that lesion diameter of mango fruits （cv. `Hongyu'）treated by LW at con－ centration of 1.0×108 cfu/mL was only 11.67 mm， it was much lower than the control fruits of 29.63 mm， the an－ tagonistic effect was very significant. The experiment of inhibiting effect on natural disease by LW showed that anthracnose disease index of fruits treated by LW at concentration of 1.0×108 cfu/mL was only 9.2 under 25 ℃ after 20 days ， and was 9.87 under 15 ℃ after 24 days. Through the analysis of colony， cell morphology， and 26s rDNA sequence， LW was identified as Metschnikowia pulcherrima.