Abstract:Using sugarcane molasses as raw material and polyurethane foam fixed Aspergillus oryzae fermenta－ tion to carry out an experiments， explored the conditions of polyurethane foam immobilized Aspergillus oryzae and the optimal conditions of immobilized Aspergillus oryzae fermentation of molasses. The optimal conditions of immobilization was obtained though the experiment： 2 mm × 2 mm × 2 mm of the best size for polyurethane foam carrier， and 1.0 g/60 mL fermented liquid of the best filling quantity， the concentration of kojic acid could reach 15.43 g/L by using the molasses treated by immobilized enzyme fermented acid， and the highest concentration of kojic acid could reach 9.02 g/L. when the concentration of sugar in cultural medium was 80 g/L.