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投稿时间:2015-01-23
投稿时间:2015-01-23
中文摘要: 用蒽酮比色法测定大野荞(Fagopyrum Megaspartanium)不同器官中可溶性糖的含量,并采用 NBT 光化还原法、 Fenton 反应法和钼酸铵显色分光光度法研究了大野荞不同器官(茎、叶、花和种子)可溶性糖体外抗羟基自由基(·OH)、 超氧阴离子自由基(·O2-)和过氧化氢(H2O2)的能力。结果表明:大野荞不同器官可溶性糖体外均能抗·OH、·O2-和 H2O2。抗·OH 的能力为:花、种子>茎、叶;抗·O2-的能力为:花>叶、种子、茎;抗 H2O2 的能力为:花、叶>茎>种子。
Abstract:Total soluble sugar in different organs of Fagopyrum megaspartanium was extracted and determined by anthrone colorimetric method. And the capacity of scavenging·OH,·O2- and H2O2 of total soluble sugar ex- tracts from four organs (aerial stem, leaf, flower and seed) of Fagopyrum megaspartanium was also studied by means of nitro blue terra-zolium photochemica reduction, Fenton reaction and spectrophotometric assay. The results showed that total soluble sugar extracts had the capacity of resisting·OH,·O2- and H2O2. The capacity of resisting·OH was sorted as: flower, seed>aerial stem, leaf. The capacity of resisting·O2- was sorted as: flow- er>leaf, seed, aerial stem. The capacity of resisting H2O2 was sorted as: flower, leaf>aerial stem>seed.
文章编号:201607006 中图分类号: 文献标志码:A
基金项目:贵州省科技厅、 毕节市科技局及毕节学院科技联合基金 (黔科合 J 字 LKB[2013]21 号);贵州省教育厅自然科学研究项目(黔教科 2010074 号); 中央高校基本科研业务费专项资金资助项目 (XDJK2012C111);贵州省科技厅、毕节市科技局及贵州工程应用技术学院科技联合基金(黔科合 LH 字 [2014]7540 号)
Author Name | Affiliation |
DENG Lin-qiong, ZHANG Yi-zhong* | Ecological Engineering School, Guizhou University of Engineering Science, Bijie 551700, Guizhou,China |
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