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食品研究与开发:2016,37(6):112-116
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响应面法优化荸荠多糖的提取工艺
王颖1,3,王国红2,曾霞1,王一飞3
(1.广东石油化工学院 环境与生物工程学院,广东 茂名 525000;2.新乡医学院 生理学与神经生物学教 研室,河南 新乡 453003;3.暨南大学 生物医药研究开发基地,广东 广州 510632)
Optimization of Extraction Technique for Polysaccharides from Eleochairistoberosa by Responsesurface Methodology
WANG Ying1,3, WANG Guo-hong2, ZENG Xia1,WANG Yi-fei3
(1.School of Environmental and Biological Engineering,Guangdong University of Petrochemical Technology,Maoming 525000,Guangdong,China;2.Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang 453003,Henan,China;3.Biomedicine Research &Development Center, Jinan University, Guangzhou 510632,Guangdong,China)
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投稿时间:2015-03-20    
中文摘要: 以荸荠为原料,研究纤维素酶协同超声波辅助提取荸荠多糖的最佳工艺。在单因素试验基础上,利用响应面 法,以多糖提取率为响应值,建立数学模型,获得最佳工艺。通过二次回归模型响应面分析得出荸荠的最佳提取工艺条 件是超声温度 62 ℃,超声时间 21 min,超声功率 600 W,酶解温度 40 ℃,酶解时间 42 min,纤维素酶用量 174 U/g,pH 为6.0。在此最佳工艺条件下荸荠多糖提取率为 4.46 %,理论值为 4.53 %,相对偏差为 1.39 %。由响应面法优化得到的荸荠 多糖的提取工艺方便可行。
Abstract:With Eleochairistoberosa as raw material, the extraction process of Eleochairistoberosa polysaccha- ride by Cellulase-assisted ultrasonic hydrolysis method was studied. Response surface method(RSM) was used to establish the mathematical model of ultrasonic extraction of Eleochairistoberosa polysaccharides to obtain the optimum conditions based on single factor experiments. The optimum conditions obtained through RSM analysis were as follows:ultrasonic temperature 62 ℃, ultrasonic time 21 min, ultrasonic power 600 W, enzymolysis temperature 40 ℃,enzymolysis time 42 min , cellulose 174 U/g, pH6.0. Under the optimized conditions, the maximum predicted extraction efficiency of polyphenols was 4.53 %, which was close to the actual average val- ue of 4.46 % with a relative error of 1.39 %. Optimization of the extraction process by RSM of Eleochairisto- berosa Polysaccharides is convenient and feasible.
文章编号:201606026     中图分类号:    文献标志码:A
基金项目:茂名市科技计划项目(2012B01027)
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