Abstract:On the basis of single factor experiment， the microwave-assisted extraction technique of bioactive compounds of Aspergillus niger inhibitors from Kalimeris indica L. was optimized by orthogonal design. The re－ sults showed that the primary and secondary order of influence of the factors microwave -assisted extraction method for bioactive compounds of Aspergillus niger inhibitors from Kalimeris indica L. was confirmed： the ratio of solid to liquid>temperature >power >time. The optimum process condition was confirmed that the yields of crude extract was 44.62 % could be obtained when extraction power was 700 W， extraction temperature was 70 ℃， extraction time was 3 min， the ratio of solid to liquid was 1 ∶ 70 （g/mL）. Verification showed the result was authentic. Further experimental results showed that the extract had better inhibitory activity on Aspergillus niger and Penicillium. The Minimum Inhibition Concentration （MIC） for Aspergillus niger and Penicillium are both about 0.94 mg/mL， showed the extract of Kalimeris indica L. has the potential to inhibit fungal growth.