本文已被:浏览 2297次 下载 660次
投稿时间:2015-01-20
投稿时间:2015-01-20
中文摘要: 优化超声辅助提取甘草多糖的条件,并对甘草多糖的分子量和结构进行了研究。单因素实验确定最佳提取条 件为:设定超声功率 500 W 时,液料比为 20 ∶ 1(mL/g),超声时间 60 min,温度 60 ℃。应用 GC/MS 对甘草多糖进行单糖组 分分析,得出人工与野生甘草多糖的单糖组分主要是甘露糖和葡萄糖,另外还含有阿拉伯糖、核糖、木糖、半乳糖、葡萄 糖醛酸和半乳糖醛酸,说明人工与野生甘草多糖均为酸性多糖。最后利用 Sepharose CL-6B 层析柱对甘草多糖进行分离 纯化, 测得人工甘草两个多糖组分相对分子量分别为 212 ku 和 25.1 ku, 野生甘草多糖两个组分的相对分子量分别为34.1 ku 和 0.1 ku。 本研究为甘草多糖的生物活性研究和应用提供了理论基础。
Abstract:In this study, the ultrasonic assisted extraction conditions of glycyrrhiza polysaccharides were opti- mized and its molecular weight and structure were investigated. The single-factor experiment was used to deter mine the optimum conditions: ultrasonic power 500 W, liquid/solid ratio 20 ∶ 1(mL/g), ultrasonic time 60 min, temperature 60 ℃. The monosaccharide composition of glycyrrhiza polysaccharide was analyzed by GC/MS. The result showed that the artificial and wild glycyrrhiza monosaccharide components was primarily contained man- nose and glucose, also including arabinose, ribose, xylose, galactose, glucuronic acid and galacturonic acid, indicating that artificial and wild glycyrrhiza polysaccharides were acidic polysaccharides. Finally, the gly- cyrrhiza polysaccharides were purified and two fractions was obtained. The relative molecular weight of artificial glycyrrhiza polysaccharides were 212 ku and 25.1 ku, respectively, while relative molecular weight of wild gly- cyrrhiza polysaccharides were 34.1 ku and 0.1 ku, respectively. This study provides a theoretical basis for the bioactivity investigation and application of glycyrrhiza polysaccharide.
keywords: glycyrrhiza polysaccharide ultrasonic assisted extraction optimization molecular characterization
文章编号:201606017 中图分类号: 文献标志码:A
基金项目:
作者 | 单位 |
何培新1,吴双双1,林莉2,闵玉涛3,许春平1,* | 1.郑州轻工业学院 食品与生物工程学院,河南 郑州 450002;2.广西中烟工业有限责任公司技术中心, 广西 南宁 530001;3.中州大学化工食品学院,河南 郑州 450044 |
引用文本: