Abstract:A mixture sample comprised of Corn，Red adzuki bean，brown rice served as substrate. After Inonotus obliquus solid fermentation，the content of total triterpenoid，Polyphenols and polysaccharides were measured respectively. Results show that，substrate fermentation achieved the best results under the right conditions that Corn，Red adzuki bean and brown rice were mixed together by crushing，screening through mesh size of 10. The content of polysaccharides，Polyphenols reached maximum 6.27 mg/g and 22.48 mg/g respectively，but good fer鄄 mentation effect resulted in high polysaccharides consumption. At this point，the content of polysaccharides of the substrate was the lowest，10.57 mg/g. Corn can improve the growth of Inonotus obliquus much more than brown rice.