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投稿时间：2014-11-27
投稿时间：2014-11-27
中文摘要: 探究绿色木霉自絮凝固定化条件，以便提高其分泌纤维素酶的能力。首先测定固定化时间、体积、pH、葡萄糖与 蛋白胨浓度对自絮凝固定化绿色木霉分泌纤维素酶的影响，然后用正交试验设计进行优化组合。时间、体积和 pH 对自 絮凝绿色木霉分泌纤维素酶的影响均呈钟形曲线；葡萄糖对自絮凝绿色木霉分泌纤维素酶影响不大；蛋白胨对自絮凝 绿色木霉分泌纤维素酶影响较大。绿色木霉自絮凝固定化最优条件是：时间为 6 d，pH 为 6，体积为 70 mL，葡萄糖为2.25 %，蛋白胨为 1.50 %。按此条件培养自絮凝固定化的绿色木霉 72 h，分泌的纤维素酶活性为 3.55 U。
Abstract:Researching on the conditions of Trichoderma viride immobilized with self-flocculation，so we could get high cellulase activity. First，we measured the effects of time，volume，pH，glucose and peptone concentra鄄 tions on cellulase activity of self-flocculating immobilized Trichoderma viride. Then we used the orthogonal test design to obtain optimized combinations. The times，volumes and pH had a bell shaped curve effect on cellulase activity of immobilized Trichoderma viride with self-flocculating. The glucoses had small effect on cellulase ac鄄 tivity，but the peptone had great effect on cellulase activity. The best optimal conditions of self-flocculation of immobilized Trichoderma viride were 6 d of time，70 mL of volume，6 of pH，2.25 % of glucoses and 1.50 % of peptones. Under the optimized conditions，the cellulase activity could reach 3.55 U after Trichoderma viride for cultivating of 72 h.
文章编号：201605031 中图分类号： 文献标志码：A
基金项目:教育部留学回国人员科研启动基金（第 44 批）；天津市科技兴海项目（KJXH2012-14）
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