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投稿时间：2015-04-07
投稿时间：2015-04-07
中文摘要: 建立了高效液相色谱-串联质谱法测定进口猪产品中莱克多巴胺残留量。样品经乙酸铵溶液提取，加入 β-盐酸 葡萄糖醛苷酶-芳基硫酸酯酶酶解后，通过 MCX 固相萃取小柱净化，采用电喷雾离子源及多反应监测模式进行检测。莱 克多巴胺在 0.25 μg/kg～5 μg/kg 范围内的标准曲线线性关系良好，相关系数 R 大于 0.99，本方法的定量检出限为0.5 μg/kg。在 3 个浓度水平进行添加回收试验，回收率为 80.4 %～96.3 %，相对标准偏差为 0.95 %～6.23 %。
Abstract:A high Performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） was developed for determining the ractopamine residuals in imported porcine products.The sample was extracted with CH3COONH solution and hydrolyzed with mixed solution of β -glucuronidase and aryl sulfatase，purified by passing through MCX SPE column.ESI was adopted and the sample was detected by the multiple reaction moni鄄 toring mode. The concentration of ractopamine presented a good linear relationship in the concentration range of 0.25 μg/kg to 5 μg/kg，related coefficient R > 0.99，and the limit of quantitation was 0.5 μg/kg.The recoveries of three concentration levels were within 80.4 %-96.3 % and RSDs ranging from 0.95 %-6.23 %.
keywords: LC-MS/MS porcine products ractopamine
文章编号：201605030 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| CHEN Dan-ping， XIE Ying-xin， CAO Xiao-yan，YANG Fu-chun， ZHANG Bao-yuan | Foshan Entry-Exit Inspection & Quarantine Bureau， Foshan 528000， Guangdong， China |
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