Abstract:Silica was modified with AMPS， and then polymethacrylic acid was grafted onto AMPS -SiO2 surface， forming polymethacrylic acid grafted silica gel （PMAA/SiO2）. With lysozyme as template molecule and ethylene glycol dimethacrylate as crosslinker， LYZ-imprinted polymeric layer was prepared and silica surface was coated with it （LYZ-MIP-PMAA/SiO2）. The material was characterized by scanning electron microscope （SEM） and particle size analysis. Infrared spectroscopy， scanning electron microscopy and particle size determination were used to characterize LYZ-MIP-PMAA/SiO2. The adsorption properties of LYZ-MIP-PMAA/ SiO2 on lysozyme were studied by static binding test and dynamic binding test， and its selective adsorption properties was investigated by selective binding test with bovine serum albumin as competing substrate. Results showed that the adsorption capacity of LYZ-MIP-PMAA/SiO2 on lysozyme was better than that of NIP-PMAA/ SiO2. The separation factor of LYZ-MIP-PMAA/SiO2 on lysozyme and bovine serum albumin was 1.20， showing that LYZ-MIP-PMAA/SiO2 displayed high recognition ability to the template molecule-LIN.