Abstract:Influences of cultural conditions on the production of antioxidant materials by the endophytic fungal Chaetomium globosum BJEF13 isolated from Illicium verum were studied. After screening the basic culture medium， influences of compositions of the cultural medium and fermentation conditions to the dry weight of mycelia and DPPH· scavenging activity were investigated using single factor experiment. The basic culture medium screening results indicated that Czapek culture medium was more suitable for the strain than the other three media， the PD culture medium， Martin culture medium and BSS culture medium. The results of single factor experiments for components of Czapek culture medium indicated that the suitable cultural conditions for the mycelia growth and production of antioxidant materials were as followed： 4 % glucose， 0.4 % peptone， 0.1 % K2HPO4， 0.05 % KCl， 0.05 % MgSO4， 0.001 % FeSO4， initial pH 7.5， temperature 28 ℃， inoculation amount 10 %， liquid volume 100 mL/250 mL， shaking speed 150 r/min and cultural time 7 days. Under the suitable cultural conditions， the dry weight of mycelia and DPPH· scavenging activity of 5 -fold dilution of acetate extract of the broth were 0.682 6 g/100 mL and 57.51 %， respectively.