Abstract:Analyze several widely-applied molecular biological detection techniques of Cronobacter sakazakii， to find the rapid and exact detection method of Cronobacter sakazakii that will meet the food laboratory testing requirements. Compare the detection sensitivity of the methods like cross priming amplification method （CPA）， real -time fluorescent PCR method， loop -mediated isothermal amplification method （LAMP） by the DNA detection sensitivity and sample added bacteria experiment； through the sample testing， compare the sensitivity， specificity， false positive rate， false negative rate and the relative accuracy based on conventional cultivation and biochemical identification method. DNA detection sensitivity showed real-time fluorescent PCR method was the most sensitive， low detection limit was 0.9 fg/test； in the sample add bacteria experiment， three methods to detect the low limit of up to 100 cfu/100 g； sensitivity compared with reference methods， three methods were 100 %， false negative rate of 0 %， the specificity was 98.8 %-99.0 %， false positive rate was 1.0 %-1.2 %， relative accuracy of 98.8 %-99.0 %， all of which can meet the demand of food laboratory testing. Compared to the benchmark method， the testing time of three kinds of molecular biology method testing was shorter， the test results were nearly accordance with the traditional standard detection method， no false negative happened， false positive hardly happened. It presented that the three methods can be used for daily Cronobacter sakazakii early screening test.