Abstract:The study was to investigate an optimal Folin -Ciocalteu colorimetric method to determine polyphenols in Dried Jujubes systematically， and to provide an effective way to estimate the polyphenols for the value of Dried Jujubes. Gallic acid was used as a standard substance to determine total polyphenol contents in different parts of Dried Jujubes growing in Xinjiang by Folin-Ciocaheu Colorimetry. With the content of total phenols as index，effects of solvent concentration，solid -liquid ratio extraction time and times on extraction technology of total phenols were investigated by L9（34） orthogonal test，in order to define preparation method of the test solution for determination. The results showed that when the concentration of Folin- Ciocalteu reagent was 1.5 mL and the concentration of 25 %Na2CO3 was 2 mL， reaction 60 min in 30 ℃， there was a good linearity relationship between absorption value with the concentration from 0-100 μg/mL in 760 nm （R2 = 0.998 8））. The total phenol in Dried Jujubes in 1 ∶ 10 （g/mL） to 50 % methanol for 30 min would with two times be preferably extracted. The average spiked recovery was 98.79 %，with a RSD of 1.71 %．The extraction and determination methods were suitable for the determination of total phenol in Jujubae Fruetus samples．The same total phenol contents differences between the pulp and core is larger， pulp total phenol content is about 6 times of core of the fruit.