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投稿时间:2015-09-07
投稿时间:2015-09-07
中文摘要: 初步研究金银花不同提取物的抗氧化活性。将金银花药材用石油醚脱脂后,分别用无水乙醇、95 %乙醇、90 % 乙醇、80 %乙醇、70 %乙醇、60 %乙醇和 50 %乙醇提取,采用紫外-可见分光光度法对不同溶剂提取物的绿原酸、总黄酮 和总酚含量进行分析,并测试各提取物对羟基自由基(·OH)和二苯代苦味酰基自由基(DPPH·)的清除作用以对其抗氧 化活性进行比较。结果表明:金银花 95 %乙醇提取物绿原酸、总黄酮和总酚含量最高,分别为 123.99、242.62 和 48.76 mg/g;
95 %乙醇提取物清除·OH 和 DPPH·能力也最强,清除率分别为 90.69 %和 65.64 %。可见,金银花清除·OH 和 DPPH·自 由基的能力与绿原酸、黄酮和多酚的含量具有一定的相关性。
中文关键词: 金银花 抗氧化活性 绿原酸 总黄酮 总酚 羟基自由基(·OH) 二苯代苦味酰基自由基(DPPH·)
Abstract:The antioxidant bioactivity of different extracts of the flower buds of Lonicera japonica was studied preliminarily in this paper. After extraction by petroleum ether, 100 % ethanol, 95 % ethanol, 90 % ethanol,80 % ethanol, 70 % ethanol, 60 % ethanol and 50 % ethanol was used to extract the flower buds of Lonicera japonica. The ultraviolet-visible spectrophotometry was applied to test the contents of chlorogeinc acid, total flavonoids and total polyphenols. To compare the antioxidant bioactivity of different extracts of the flower buds of Lonicera japonica,·OH and DPPH·free radical scavenging ability were measured as well. The results showed that 95 % ethanol extract had highest content of chlorogeinc acid, total flavonoids and total polyphenols, that was 123.99 mg/g, 242.62 mg/g and 48.76 mg/g; 95 % ethanol extract had the strongest·OH and DPPH·radical scavenging ability with scavenging ratio of 90.69 % and 65.64 %. Thus,·OH and DPPH·scavenging capacity presented probably positive relatively with concentration of chlorogeinc acid, total flavonoids and total polyphenols.
keywords: the flower buds of Lonicera japonica antioxidant bioactivity chlorogenic acid total flavonoids total polyphenols hydroxyl radical (·OH) diphenyl picryl hydrazinyl radical (DPPH·)
文章编号:201601013 中图分类号: 文献标志码:A
基金项目:国家自然科学基金地区项目(31160332);内蒙古自治区高 等学校科学技术研究项目(NJZZ13015);内蒙古大学高层次人才引进 科研启动项目(Z20100108)
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