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投稿时间:2026-01-23
投稿时间:2026-01-23
中文摘要: 为提升大豆亲脂性蛋白(soybean lipophilic protein,SLP)在充气食品领域的应用潜力,该研究以SLP与果胶(pectin,PC)为原料,探究SLP与PC不同混合质量比对复合物理化特性、结构及起泡性能的影响。对SLP-PC复合物的微观形貌、平均粒径与Zeta电位、内源荧光光谱、傅里叶变换红外光谱、表面疏水性、界面张力及起泡特性进行分析,并观察泡沫的微观结构。结果表明,与单一SLP相比,当SLP与PC质量比为4∶1时,复合物的起泡性显著提升至125%,泡沫稳定性提高至43.6%。结构分析表明,PC通过氢键、静电及疏水相互作用与SLP结合,诱导蛋白质去折叠,使其二级结构由有序向无序转变,导致埋藏的疏水基团暴露,表面疏水性显著增加。同时,复合物在SLP与PC质量比4∶1下粒径最小,表面疏水性及界面活性最高,有利于其在空气-水界面快速吸附并显著降低界面张力,从而在界面处构建一层厚实且具有高黏弹性的复合膜。综上,PC可有效调控SLP的结构特征并强化其界面性能。
Abstract:To enhance the application potential of soybean lipophilic protein (SLP) in the field of aerated foods,this study used SLP and pectin (PC) as raw materials to investigate the effects of different mixing ratios of SLP and PC on the physicochemical properties,structural characteristics and foaming performance of the composite system. The microscopic morphology,average particle size,Zeta potential,intrinsic fluorescence spectroscopy,Fourier transform infrared spectroscopy(FTIR),surface hydrophobicity,interfacial tension and foaming properties of the SLP-PC composites were analyzed,and the microstructure of the foams was observed.The results showed that compared with pure SLP,when the mass ratio of SLP to PC was 4∶1,the foaming capacity of the composite was significantly increased to 125%,and the foaming stability was improved to 43.6%.Structural analysis indicated that PC bound to SLP through hydrogen bonding,electrostatic and hydrophobic interactions,which induced protein unfolding,triggered the transition of the protein secondary structure from an ordered to a disordered conformation,and resulted in the exposure of buried hydrophobic groups and a significant increase in surface hydrophobicity. Meanwhile,the composite at the SLP and PC mass ratio of4∶1 had the smallest particle size,the highest surface hydrophobicity and the optimal interfacial activity. These properties facilitated its rapid adsorption at the air-water interface and remarkable reduction of interfacial tension,thereby constructing a thick composite film with high viscoelasticity at the interface. In summary,PC can effectively regulate the structural characteristics of SLP and enhance its interfacial properties.
keywords: soybeanlipophilic protein pectin non-covalentinteractions air-waterinterface foaming properties
文章编号:202617011 中图分类号: 文献标志码:
基金项目:
| 作者 | 单位 |
| 贾智宇 | 东北农业大学食品学院,黑龙江哈尔滨 150030 |
| 陈薪诺 | 东北农业大学食品学院,黑龙江哈尔滨 150030 |
| 杨颜如 | 东北农业大学食品学院,黑龙江哈尔滨 150030 |
| 宋嘉宝 | 东北农业大学食品学院,黑龙江哈尔滨 150030 |
| 徐正 | 东北农业大学食品学院,黑龙江哈尔滨 150030 |
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