Abstract:This study aimed to explore functional components and optimal extraction parameters of ginsengginger-jujube decoction against gastric ulcer. Liquid chromatography-tandem mass spectrometry was used to identify chemical compositions of the decoction. Targets of chemical ingredients were predicted via the traditional Chinese medicine systems pharmacology database and analysis platform （TCMSP） database，and gastric ulcer-related targets were retrieved from the PubChem database. Core targets and associated signaling pathways were analyzed using STRING and DAVID databases. A network of active component-target-signaling pathway was established with Cytoscape 3.10.1. Functional factors were screened based on degree values，and molecular docking was performed to verify binding affinity between core components and key targets. Singlefactor and orthogonal experiments were conducted to optimize extraction conditions with total flavonoid content and extraction yield as evaluation indicators.The results indicated that 38 compounds were successfully identified，consisting of 18 flavonoids，8 saponins，4 organic acids，2 saccharides and 6 other substances. Network pharmacological analysis demonstrated that flavonoids including isovitexin，isoliquiritigenin，wogonin，rutin and liquiritin served as predominant functional factors. These ingredients targeted core genes AKT1，TNF，MMP9，NFKB1 and TP53，and relieved gastric ulcer symptoms by modulating vascular endothelial growth factor signaling pathway （VEGF） and epidermal growth factor receptor signaling pathway （EGFR）. Orthogonal tests confirmed the optimal extraction conditions：liquid-solid ratio 120∶1（mL/g），soaking duration 40 min，single extraction duration 60 min，and two extraction cycles. Under the optimized conditions，the total flavonoid content was 14.7 mg/g，and the extraction yield reached 39.78%.