Abstract:Artificially cultured Andrias davidianus has high nutritional and medicinal values. At present，product development mainly focuses on Andrias davidianus meat products，while there is a lack of high-value development and utilization of its protein-rich skin and mucus. In this study，Andrias davidianus protein was extracted from the skin and mucus of Andrias davidianus by alkali extraction and acid precipitation method，and Andrias davidianus protein peptides were prepared by compound protease hydrolysis technology. Their antioxidant and anti-fatigue activities were evaluated through in vitro and in vivo experiments on mice. In vitro experiments showed that the scavenging rates of Andrias davidianus protein peptides （5 mg/mL） on superoxide anion radicals，hydroxyl radicals，1，1-diphenyl-2-picrylhydrazyl （DPPH） radicals，and 2，2′-azinobis-（3-ethylbenzothiazoline-6-sulphonate） （ABTS） cation radicals were 69.09%，43.69%，61.20%，and 82.79%，respectively.Its antioxidant activity was comparable to that of glutathione （GSH） and was concentration-dependent. In vivo experiments on mice indicated that the liver glycogen contents of mice fed with Andrias davidianus skin protein peptides and mucus protein peptides were increased by 23% and 18%，respectively，compared with the control group. Meanwhile，the activities of plasma lactate dehydrogenase in the two groups reached 140 U/mL and 160 U/mL，respectively，which were significantly higher than those in the control group. The content of urea nitrogen in plasma was decreased by 20.0%-22.5%，and the content of blood lactic acid after exercise was decreased by 10%-14%. These results suggested that Andrias davidianus protein peptides could accelerate the metabolism of lactic acid and urea nitrogen and alleviate exercise-induced muscle damage and fatigue.