Abstract:Most nutrients， such as polyunsaturated fatty acids， polyphenols， and vitamins， suffer from poor stability， low solubility， unsatisfactory oral bioavailability， and production of undesirable flavors， which limit their direct application in foods. Therefore， construcing efficient and stable delivery systems has become a key strategy to protect nutrients and enhance their biological activities. Owing to their excellent nutritional value，biocompatibility， and versatile functional properties， proteins and polysaccharides have become ideal materials for the construction of food-grade nutrient delivery systems. The recent research progress in nutrient delivery systems based on protein-polysaccharide complexes was systematically reviewed. The main preparation methods of protein-polysaccharide complexes were described in detail， and the principles， advantages， limitations， and application scenarios of different methods were analyzed and compared. Furthermore， various delivery systems based on protein-polysaccharide complexes， including emulsions， gels， and nanoparticles were systematically summarized， together with their performance in improving nutrient stability， encapsulation efficiency， bioaccessibility， and controlled-release capacity. The key factors affecting the performance of these delivery systems were thoroughly discussed. Finally， the current applications of protein-polysaccharide complexbased delivery systems in dietary supplements， food ingredients， food packaging， and food detection were reviewed， and their potential applications in emerging fields were proposed， including cultured meat scaffolds，plant-based meat analogs， 3D printing ， “three （salt， oil， and sugar）-reduced” foods， and stimuli-responsive delivery systems.