Abstract:This paper aims to investigate the effects of Cistanche deserticola fermented tea on the immune function and gut microbiota in cyclophosphamide-induced immunosuppressed mice.An immunosuppressed mouse model was built by adopting cyclophosphamide induction，followed by the administration of C.deserticola fermented tea.Meanwhile，changes in the thymus index，pathological morphology of the spleen，and lymphocyte subset levels were measured，and high-throughput sequencing technology was employed to analyze the intestinal microbiota in mouse feces.Result showed that C.deserticola fermented tea could enhance the immune function in immunosuppressed mice，significantly increased the thymus index，alleviate spleen pathological damage，and increased the percentage of CD3+ T cells，B cells，and CD4+∕CD8+ ratio，thus exerting immunoregulatory effects.C.deserticola fermented tea also alleviates gut microbiota dysbiosis，significantly increasing the relative abundance of probiotics such as Prevotella，Akkermansia，and Alloprevotella，thereby enhancing its immunoregulatory ability.C.deserticola fermented tea can enhance the immune function of cyclophosphamideinduced immunosuppressed mice，and the mechanism may be closely related to the regulation of gut microbiota balance.