Abstract:In this study，Cenchrus fungigraminus juice was used as the medium of Lactobacillus plantarum FJAT-54898. The results indicated that after anaerobic fermentation at 30 ℃ for 36 h，the C. fungigraminus content reached 1.9×109 cfu/mL，with a dynamic equation of content change being y = 5×106x3 - 3×107x2 + 7× 107x - 4×107 （R2 = 0.998 7）. The C. fungigraminus fermentated by L. plantarum FJAT-54898 was rich in nutrients and minerals，including protein （0.42 g/100 g），total nitrogen （0.67 mg/g），total polyphenols（0.19%），total sugars （7.10 g/100 g），total acids （2.62 g/kg），total flavonoids （0.09%），vitamin C （9.40 mg/100 g），zinc （15.90 mg/100 g），and iron （32.70 mg/100 g）. Seventeen amino acids were detected with a total content of 0.227 g/100 g，and the top five amino acids of high content were alanine （0.042 g/100 g），glutamic acid （0.028 g/100 g），aspartic acid （0.023 g/100 g），lysine （0.016 g/100 g），and leucine （0.016 g/100 g）.In conclusion，the fermentation broth had broad prospects for the development of aquatic，livestock，poultry，and plant probiotics.