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复合酶酶解中国毛虾制备生物活性肽
相卜瑗1，2，邵如雪1，2，曹家鸣1，2，程钰1，2，亢新鑫1，2，王淑军1，2 *
(1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005)
Preparation of Bioactive Peptides by Hydrolysis of Acetes chinensis with Composite Enzymes
XIANG Boyuan1,2,SHAO Ruxue1,2,CAO Jiaming1,2,CHENG Yu1,2,KANG Xinxin1,2,WANG Shujun1,2*
(1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China)
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投稿时间：2024-03-23
中文摘要: 该研究以中国毛虾（Acetes chinensis）为原料，酶解制备具有抗氧化活性和抑制α-葡萄糖苷酶的生物活性肽。在单酶酶解的基础上，进行复合酶酶解，检测酶解产物的抗氧化活性和α-葡萄糖苷酶抑制率。结果表明，最适酶解条件为胰蛋白酶与糖化酶复合，酶解温度45 ℃、pH7、酶添加量3%、料液比1∶8（g/mL）、酶解时间 4 h，此时，毛虾粗肽α-葡萄糖苷酶抑制率达到87.06%。酶解液经超滤、葡聚糖凝胶柱分离纯化和液相-质谱联用技术鉴定后，得到6条具有抗氧化活性和α-葡萄糖苷酶抑制作用的多肽，氨基酸序列分别为 GHYTEGAELVDSVLDVVRK、NVLIFDLGGGTFDVSILTIEDGIFEVK、NSWGTGW、WGTGW、EGSGTGEG和EGDATGAT。
Abstract:Acetes chinensis was hydrolyzed with enzymes to prepare bioactive peptides with antioxidant and inhibitory effects on α-glucosidase.On the basis of single enzyme hydrolysis,composite enzyme hydrolysis was carried out to test the antioxidant activity and inhibition rate of the products on α-glucosidase.The results showed that the optimal enzymatic hydrolysis conditions were the combination of papain with glucoamylase,reaction temperature of 45 ℃,pH of 7,enzyme addition of 3%,solid-liquid ratio of 1∶8（g/mL）,and enzymatic hydrolysis time of 4 h.The inhibition rate of α-glucosidase in the crude peptide of Acetes chinensis reached 87.06%.After purification of ultrafiltration and Sephadex G25 separation,the hydrolysate was identified by liquid chromatography-mass spectrometry,and six strains with antioxidant activity and inhibitory effects on αglucosidase were obtained.The amino acid sequences were GHYTEGAELVDSVLDVVRK,NVLIFDLGGGTFDVSILTIEDGIFEVK,NSWGTGW,WGTGW,EGSGTGEG,and EGDATGAT,respectively.
文章编号：202605013 中图分类号： 文献标志码：
基金项目:国家重点研发计划项目（2022YFC2805101）；连云港市“521工程”科研资助项目（LYG06521202107）
| 作者 | 单位 |
| 相卜瑗 | 1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005 |
| 邵如雪 | 1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005 |
| 曹家鸣 | 1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005 |
| 程钰 | 1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005 |
| 亢新鑫 | 1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005 |
| 王淑军 | 1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室,江苏 连云港 222005；2.江苏海洋大学 江苏省海洋生物技术重点实验室,江苏 连云港 222005 |
| Author Name | Affiliation |
| XIANG Boyuan | 1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China |
| SHAO Ruxue | 1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China |
| CAO Jiaming | 1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China |
| CHENG Yu | 1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China |
| KANG Xinxin | 1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China |
| WANG Shujun | 1.Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China；2.Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China |
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